HP4D - Drying Process - Climate-KIC Start-Ups
In parallel, the plasmid hp4d-tll was double digested with Sal I/ Bgl II to isolate the four-UAS1B sequence. Then, pENOn and four-UAS1B were ligated into Sal I/ Hind III-digested hp4d-tll, replacing the mLeu2 promoter to form hp4e n-tll (n = 100, 125, 200, 400, 700, 1000, and 1300 bp), respectively .
Co-expression of heterologous desaturase genes in
This study aimed to over-express the LRO1 gene driven by hp4d or TEFintron promoters to explore the outcome in biomass and lipid production. The hp4d promoter contains four tandem copies of an upstream activator sequence of a UAS1B fragment from pXPR2 and a minimal pLEU2 fragment.
HP4D: Situs Judi Slot Online Pay4d Gacor Paling Banyak Dicari
Indeed, several strong synthetic hybrid promoters have been created by fusing tandem repeats of upstream activation sequence (UAS) upstream to a core promoter region. The first one (hp4d) was based on four tandem repeats of the 108 bp UAS1 XPR2 of the XPR2 gene upstream on the minimal LEU2 core promoter .
Using a vector pool containing variable-strength promoters to ... - PubMed
the artificial hp4d promoter, when grown in both complete and low-cost industrial ingredient (10% fish and sunflower meal) containing media. The new expression system may greatly expand the range of recombinant producers of feed enzymes and some other types of fodder additives in the Y. lipolytica yeast, appropriate for assimilation of low-cost
Protein expression and secretion in the yeast Yarrowia lipolytica
The hp4d promoter expression cassette in an expression/secretion vector has been applied to produce or secrete significant levels of various recombinant proteins in previous reports (reviewed in Ref. [5]). We constructed a novel plasmid with two cassettes in order to co-express two heterologous genes simultaneously.
Figure 1: Construction of the recombinant expression vector...
In order to generate plasmid pKG2-PIS, hp4d-hph-LIP2t of plasmid pKG1-PIS was replaced by the orotidine 5′-phosphate decarboxylase expression cassette from Y. lipolytica (URA3p-URA3-URA3t ...
New inducible promoter for gene expression and synthetic biology in
As shown in Figure 3, hp4d was much more carrying a translational fusion of either minimal pLEU2 efficient than either pXPR2 or pLEU2, for protein production (pINA1208), hp4d (pINA1209, shown in Figure 1), or hp4i in both rich or minimal media.
Part:BBa K1592004 - parts.igem.org
The yeast Y. lipolytica is a dimorphic organism that can produce alkaline extracellular protease and lipase. This article describes the development of host strains and vectors for expressing and secreting heterologous proteins using the hp4d promoter, a synthetic promoter based on the HP4D gene.
Construction of pINA1296-GOX vector. hp4d: hybrid ... - ResearchGate
Promoter hp4d is composed of four tandem repeated UAS1 sequences in upstream and the small LEU2 promoter, which is the sequence between TATA box and start condon ATG of LEU2 promoter in downstream. UAS1 is the upsteam activating sequence of the pXPR2, which is less affected by other conditions.
Using a vector pool containing variable-strength promoters to optimize ...
The VDAC-driven clones exhibited high variability of the expression profile. Some clones were more active than the clones induced by the artificial hp4d promoter, when grown in both complete and low-cost industrial ingredient (10% fish and sunflower meal) containing media.